Ask for quantified cell lysate

Ask for the tris base

weight themilk down there

Check the Gell running buffer

aliquot HCL

dont forget the T-MED

1.5 M Tris-HCl, pH 8.8 (50 ml)

• Tris base 9.07 g
• diH2O 40 ml
• Adjust to pH 8.8 with 6 N HCl
• diH2O to 50

0.5 M Tris-HCl, pH 6.8 (50 ml)

• Tris base 3 g
• diH2O 40 ml
• Adjust to pH 6.8 with 6 N HCl
• diH2O to 50

10% (w/v) APS (fresh daily)

• Ammonium persulfate 0.10 g
• diH2O 1 ml

10x TBS